MaxVisionTM /AP Immunohistochemical Staining Procedure
The ready-to-use rapid immunohistochemistry MaxVisionTM/AP kit is based on polymer technology, which conjugates alkaline phosphatase with anti-mouse or anti-rabbit IgG molecules onto a polymer to form polymer molecules. The staining procedure is as follows:
1. Deparaffinize and hydrate paraffin sections, rinse with tap water;
2. Perform appropriate antigen retrieval on the tissue according to the requirements of the primary antibody;
3. Remove PBS, apply the primary antibody onto the sections, incubate at room temperature for 60 minutes or at 4°C overnight, rinse with PBS three times, 3 minutes each time (3 × 3 minutes);
4. Remove PBS, apply MaxVisionTM/AP reagent onto the sections, incubate at room temperature for 15 minutes. Rinse with PBS 3 × 3 minutes;
5. Remove PBS, apply BCIP/NBT (or AP-Red) chromogenic reagent onto the sections for color development;
6. Rinse with distilled water to stop color development, counterstain with nuclear fast red (or hematoxylin), and mount with an aqueous mounting medium.