MaxVisionTM 2/HRP Immunohistochemistry Staining Procedure
The ready-to-use rapid immunohistochemistry MaxVisionTM 2/HRP kit is a new product developed from MaxVisionTM/HRP. It is formed by polymer technology, which combines horseradish peroxidase with anti-mouse or/and anti-rabbit IgG molecules on a polymer to create polymer molecules. The staining steps are as follows:
1. Deparaffinize and hydrate paraffin sections, rinse with tap water;
2. Perform appropriate antigen retrieval on the tissue according to the requirements of the primary antibody;
3. If necessary (tissue contains endogenous peroxidase), add peroxidase blocking reagent to the section, incubate at room temperature for 10 minutes, rinse with PBS 3 times, 3 minutes each time (3×3 minutes);
4. Remove PBS, add primary antibody to the section, incubate at room temperature for 60 minutes or overnight at 4°C, rinse with PBS 3×3 minutes;
5. Remove PBS, add MaxVisionTM 2/HRP reagent to the section, incubate at room temperature for 15 minutes. Rinse with PBS 3×3 minutes;
6. Remove PBS, add freshly prepared enhanced DAB chromogenic reagent to the section for color development;
7. Rinse with tap water to stop color development, counterstain with hematoxylin, (if necessary, differentiate with 1% hydrochloric acid ethanol), return to blue with PBS, dehydrate through graded ethanol, clear with xylene, and mount with neutral resin.