High Temperature and High Pressure Antigen Retrieval Method with Citric Acid Antigen Retrieval Solution (Highly Recommended)

2. Required Reagents: Citric acid antigen retrieval solution (Maixin product number: MVS-0100/0101 or MVS-0066), distilled water.

3. Operating Steps:

(1) Deparaffinize and hydrate paraffin sections, rinse with tap water and soak in water for later use;

(2) Take an appropriate amount of diluted citric acid antigen retrieval solution (800-1500mL) as working solution into the pressure cooker, place the pressure cooker on the induction cooker, and heat at high power until boiling;

(3) Adjust the power to medium (800-1000W), place the sections on the high-temperature resistant staining rack, put them into the boiling retrieval solution, cover the pot lid, fasten the pressure valve, and continue heating;

(4) When the pressure valve starts to release steam, time for 1.5-2 minutes, then remove the pressure cooker from the induction cooker. Allow it to cool naturally at room temperature for 10 minutes. Tap water can be sprayed on the outer wall of the pressure cooker to accelerate cooling of the retrieval solution inside. After cooling to room temperature, remove the sections and rinse thoroughly with tap water. (It is recommended to remove tap water and draw a circle 3mm away from the tissue using an immunohistochemistry pen (Maixin product number: PEN-0002)). Rinse with PBS 2×3 minutes, then proceed according to the operating steps of the relevant Maixin kit.