Direct Boiling Antigen Retrieval Method with EDTA Antigen Retrieval Solution

2. Required Reagents: EDTA antigen retrieval solution (Maixin product number: MVS-0098/0099), distilled water.

3. Operating Steps:

(1) Deparaffinize and hydrate paraffin sections, rinse with tap water and soak in water for later use;

(2) Take an appropriate amount of EDTA antigen retrieval solution diluted to working concentration into the stainless steel pot, place the pot on the induction cooker, and heat at high power until boiling;

(3) Adjust the power to the minimum (maintaining a simmer), place the sections on the high-temperature resistant staining rack, put them into the boiling retrieval solution, cover the pot, and continue heating for 20 minutes;

(4) Remove the stainless steel pot from the induction cooker, allow it to cool naturally at room temperature for 10 minutes. Tap water can be poured over the outer wall of the pot to accelerate cooling of the retrieval solution inside. After cooling to room temperature, remove the sections, rinse thoroughly with tap water, (it is recommended to remove tap water and draw a circle around the tissue at a distance of 3mm using an immunohistochemistry pen (Maixin product number: PEN-0002)), rinse with PBS 2×3 minutes, then proceed according to the operating steps of the relevant Maixin kit.