Direct Boiling Antigen Retrieval Method with Citric Acid Antigen Retrieval Solution

2. Required Reagents: Citric acid antigen retrieval solution (Maixin product number: MVS-0100/0101 or MVS-0066), distilled water.

3. Operating Steps:

(1) Dewax and hydrate paraffin sections, rinse with tap water and soak in water for later use;

(2) Take an appropriate amount of diluted citric acid antigen retrieval solution (working solution) into the stainless steel pot, place the pot on the induction cooker, and heat at high power until boiling;
(3) Adjust the power to the minimum (maintaining a heat preservation state), place the sections on the high-temperature resistant staining rack, put them into the boiling retrieval solution, cover the pot, and continue heating for 20 minutes;

(4) Remove the stainless steel pot from the induction cooker, allow it to cool naturally at room temperature for 10 minutes. Tap water can be sprayed on the outer wall of the pot to accelerate cooling of the retrieval solution inside. After cooling to room temperature, remove the sections and rinse thoroughly with tap water. (It is recommended to remove tap water and draw a circle around the tissue at a distance of 3mm using an immunohistochemistry pen (Maixin product number: PEN-0002)). Rinse with PBS 2×3 minutes, then proceed according to the operating steps of the relevant Maixin kit.